Product Datasheet  
ATG4A Antibody  
Catalog Number: 34631  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q8WYN0
    NCBI Gene ID: 115201
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized peptide derived from C-terminal of human ATG4A.
  • reactivity：  
  • Hu Ms
  • appl_detail：  

  • Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:500
  • other_names：  
  • Cysteine protease ATG4A; EC 3.4.22.-; Autophagy-related protein 4 homolog A; hAPG4A; Autophagin-2
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total ATG4A protein.
  • Applications：  
  • IHC IF
  • Background：  
  • Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

    Marino G., J. Biol. Chem. 278:3671-3678(2003).
    Kabeya Y., J. Cell Sci. 117:2805-2812(2004).
    Ross M.T., Nature 434:325-337(2005



 
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