Product Datasheet  
TOP2A Antibody  
Catalog Number: 33671  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P11388
    NCBI Gene ID: 7153
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized peptide derived from N-terminal of human TOP2A.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Western blotting: 1:500~1:3000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:500
  • other_names：  
  • DNA topoisomerase 2-alpha; EC 5.99.1.3; DNA topoisomerase II; alpha isozyme; TOP2A
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total TOP2A protein.
  • Applications：  
  • WB IHC IF
  • Background：  
  • Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

    Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988).
    Wasserman R.A., Cancer Res. 53:3591-3596(1993).
    Lang A.J., Gene 221:255-266(1998).



 
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