Product Datasheet  
TOP3B Antibody  
Catalog Number: 33641  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: O95985
    NCBI Gene ID: 8940
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized peptide derived from internal of human TOP3B.
  • reactivity：  
  • Hu Ms
  • appl_detail：  
  • Western blotting: 1:500~1:3000

  • other_names：  
  • DNA topoisomerase 3-beta-1; EC 5.99.1.2; DNA topoisomerase III beta-1; TOP3B; TOP3B1
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total TOP3B protein.
  • Applications：  
  • WB
  • Background：  
  • Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Possesses negatively supercoiled DNA relaxing activity.

    Ng S.-W., Nucleic Acids Res. 27:993-1000(1999).
    Kawasaki K., Genome Res. 7:250-261(1997).
    Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).



 
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