Product Datasheet  
PCNA Antibody  
Catalog Number: 33468  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P12004
    NCBI Gene ID: 5111
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized peptide derived from human PCNA.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Western blotting: 1:500~1:3000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • cyclin; proliferating cell nuclear antigen;
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total PCNA protein.
  • Applications：  
  • WB IHC
  • Background：  
  • Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

    Anje Sporbert, Nucleic Acids Res., Jun 2005; 33: 3521 - 3528.
    Sean C. Gehen, Am J Physiol Lung Cell Mol Physiol, Mar 2007; 292: L716 - L724.
    Basavaraj Bagewadi, J. Virol., Nov 2004; 78: 11890 - 11903.
    T Iida, I Suetake, Genes Cells, Oct 2002; 7: 997 -



 
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