Product Datasheet  
IκB-α (Ab-32/36) Antibody  
Catalog Number: 33192  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P25963
    NCBI Gene ID: 4792
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized non-phosphopeptide derived from human IκB-α around the phosphorylation site of Serine 32/36.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Western blotting: 1:500~1:3000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total IκB-α protein.
  • Applications：  
  • WB IHC
  • Background：  
  • Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

    Mattioli I, et al. (2004)J Immunol; 172(10): 6336-44.
    Courtois G, et al. (2003)J Clin Invest; 112(7): 1108-15.
    Nair A, et al. (2003) Oncogene; 22(1): 50-8.
    Fan C, et al. (2002)J Cell Sci; 115(Pt 24): 4843-53.
    Schubert SY, et al. (2002)FASEB J; 16(14): 193



 
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