Product Datasheet  
PAK1/2/3 (Ab-423/402/421) Antibody  
Catalog Number: 33140  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q13153/Q13177/O75914
    NCBI Gene ID: 5058/5062/5063
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized non-phosphopeptide derived from human PAK1/2/3 around the phosphorylation site of threonine 423/402/421 (R-S-T(p)-M-V).
  • reactivity：  
  • Hu
  • appl_detail：  
  • Western blotting: 1:500~1:3000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • EC 2.7.11.1; Gamma-PAK; P21-activated kinase 2; PAK 2; PAK-2
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total PAK1/2/3 protein.
  • Applications：  
  • WB IHC
  • Background：  
  • Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.

    Bernadett Boda, J. Neurosci., Dec 2004; 24: 10816 - 10825.
    Donna L. McPhie, J. Neurosci., Jul 2003; 23: 6914.
    Jin-Hun Jung, J. Biol. Chem., Dec 2005; 280: 40025 - 40031.
    Guo-Lei Zhou, Mol. Cell. Biol., Nov 2003; 23: 8058 - 8069.



 
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