Product Datasheet  
NPHS1 Antibody  
Catalog Number: 31249  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot:P30989
    Gene ID:4923
  • Form of Antibody：  
  • Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Synthetic peptide peptide corresponding to a region derived from 1229-1241 amino acids of human nephrosis 1, congenital, Finnish type (nephrin)
  • reactivity：  
  • Hu Ms
  • appl_detail：  
  • Predicted MW: 135kd
    ELISA: 1:2000-1:5000
    Western blotting: 1:500-1:2000
    Immunohistochemistry: 1:25-1:100
  • other_names：  
  • nephrosis 1, congenital, Finnish type (nephrin), CNF, NPHN, nephrin
  • Purification：  
  • Specificity：  
  • The antibody detects endogenous level of total NPHS1 protein.
  • Applications：  
  • ELISA WB IHC
  • Background：  
  • This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in transfected COS-7 cell lysates.



 
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