RPA 14 kDa subunit antibody

Catalog Number:

22947

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P35244
Gene ID:6119

Form of Antibody:

Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

Storage/Stability:

Immunogen:

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 91 of RPA 14 kDa subunit

Purification:

Purified by antigen-affinity chromatography.

Specificity/Sensitivity:

Applications:

WB IHC

Background:

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

References:

appl_detail:

Predicted MW: 14kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250

Datasheet PDF