E2F1 antibody

Catalog Number:

22881

Amount:

100μg

Price:

$319

Swiss-Prot No:

NCBI Gene ID: 1869
NCBI mRNA#: NM_005225
NCBI Protein#: NP_005216

Form of Antibody:

Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

Storage/Stability:

Immunogen:

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 133 and 322 (Q01094) of E2F1

Purification:

Purified by antigen-affinity chromatography.

Specificity/Sensitivity:

Applications:

WB IF

Background:

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq]

References:

appl_detail:

Predicted MW: 47kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200

Datasheet PDF