Pyruvate Dehydrogenase E1 alpha antibody

Catalog Number:

22804

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P08559
Gene ID:5160

Form of Antibody:

Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

Storage/Stability:

Immunogen:

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 39 and 295 of Pyruvate Dehydrogenase E1 alpha

Purification:

Purified by antigen-affinity chromatography.

Specificity/Sensitivity:

Applications:

WB IF

Background:

The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

References:

appl_detail:

Predicted MW: 43kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200

Datasheet PDF