NDUFA5 antibody

Catalog Number:

22423

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:Q16718
Gene ID:4698

Form of Antibody:

Supplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

Storage/Stability:

Immunogen:

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 86 of Human NDUFA5

Purification:

Purified by antigen-affinity chromatography.

Specificity/Sensitivity:

Applications:

WB IHC

Background:

The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq]

References:

appl_detail:

Predicted MW: 13kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500

Datasheet PDF