HIF2A Antibody

Catalog Number:

21690

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q99814; NCBI Gene ID: 2034; NCBI mRNA: NM_001430.4; NCBI Protein: NP_001421.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.547-551(P-E-E-R-L) derived from HumanHIF2A.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total HIF2A protein.

Applications:

WB

Background:

 Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

References:

appl_detail:

 

Predicted MW: 120kd

Western blotting : 1:500~1:1000

 

 

Datasheet PDF