Histone H3 (Ab-27) Antibody

Catalog Number:

21689

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P68431
NCBI Gene ID: 8350
NCBI mRNA: NM_003529.2
NCBI Protein: NP_003520.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.26-30(A-R-K-S-A) derived from Human Histone H3.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Histone H3 protein.

Applications:

WB

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

 

Choi H.S., Choi B.Y., Cho Y.-Y., Zhu F., Bode A.M., Dong Z.J. Biol. Chem. 280:13545-13553(2005)

Tan M., Luo H., Lee S., Jin F., Yang J.S., Montellier E., Buchou T., Cheng Z., Rousseaux S., Rajagopal N., Lu Z., Ye Z., Zhu Q., Wysocka J., Ye Y., Khochbin S., Ren B., Zhao Y.

Cell 146:1016-1028(2011) 

 

References:

appl_detail:

Predicted MW: 17kd

Western blotting: 1:500~1:1000

Datasheet PDF