Twist2 Antibody

Catalog Number:

21669

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q8WVJ9
NCBI Protein: NP_476527.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.26~30(P-K-R-F-G) derived from Human Twist2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Twist2 protein.

Applications:

WB

Background:

Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism. Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors.

Lee M.S., Lowe G., Flanagan S., Kuchler K., Glackin C.A.Bone 27:591-602(2000)

References:

appl_detail:

Western blotting      1:100-1:500
Immunohistochemistry  1:100-1:500

Datasheet PDF