E2F-1 Antibody

Catalog Number:

21646

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q01094
NCBI Protein: NP_005216.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.430~434(G-D-L-T-P) derived from Human E2F-1

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of total E2F-1 protein.

Applications:

WB

Background:

Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. "P53 and E2F-1 cooperate to mediate apoptosis."

Wu X., Levine A.J. Proc. Natl. Acad. Sci. U.S.A. 91:3602-3606(1994) "Regulation of E2F1 activity by acetylation."
Martinez-Balbas M.A., Bauer U.M., Nielsen S.J., Brehm A., Kouzarides T. EMBO J. 19:662-671(2000) "Regulation of E2F1 function by the nuclear corepressor KAP1."
Wang C., Rauscher F.J. III, Cress W.D., Chen J. J. Biol. Chem. 282:29902-29909(2007)

References:

appl_detail:

Predicted MW: 70kd
Western blotting: 1:500~1:1000
 

Datasheet PDF