g-Catenin Antibody

Catalog Number:

21645

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P14923
NCBI Protein: NP_002221.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.726~730(I-D-T-Y-S) derived from Human g-Catenin

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of total g-Catenin protein.

Applications:

WB

Background:

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton "Characterization of the interactions of a-catenin with a-actinin and beta-catenin/plakoglobin."

Nieset J.E., Redfield A.R., Jin F., et al. J. Cell Sci. 110:1013-1022(1997) "Interaction of the DF3/MUC1 breast carcinoma-associated antigen and beta-catenin in cell adhesion."
Yamamoto M., Bharti A., Li Y., Kufe D. J. Biol. Chem. 272:12492-12494(1997) "The transmembrane receptor protein tyrosine phosphatase DEP1 interacts with p120(ctn)."
Holsinger L.J., Ward K., Duffield B., Zachwieja J., Jallal B. Oncogene 21:7067-7076(2002)

References:

appl_detail:

Predicted MW: 83kd
Western blotting: 1:500~1:1000
 

Datasheet PDF