TIF1b Antibody

Catalog Number:

21635

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q13263
NCBI Protein: NP_005753.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.827~831 (L-S-G-G-P) derived from Human TIF1b.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total TIF1b protein.

Applications:

WB

Background:

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.

Friedman J.R., Fredericks W.J., Jensen D.E. Genes Dev. 10:2067-2078(1996)
Moosmann P.R., Georgiev O., le Douarin B., Bourquin J.-P. Nucleic Acids Res. 24:4859-4867(1996)
Agata Y., Matsuda E., Shimizu A.J. Biol. Chem. 274:16412-16422(1999)

References:

appl_detail:

Predicted MW: 110kd
Western blotting: 1:500~1:1000

Datasheet PDF