Doublecortin Antibody

Catalog Number:

21630

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O43602
NCBI Protein: NP_000546.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.413~417( P-T-S-P-G) derived from Human Doublecortin.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Doublecortin protein.

Applications:

WB

Background:

Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.

Des Portes V., Pinard J.-M., Billuart P. Chelly J.Cell 92:51-61(1998)
Pilz D.T., Matsumoto N., Minnerath S.R., Mills P. Hum. Mol. Genet. 7:2029-2037(1998)

References:

appl_detail:

Predicted MW: 40-45kd
Western blotting: 1:500~1:1000

Datasheet PDF