Ring1A Antibody

Catalog Number:

21629

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q06587
NCBI Protein: NP_002922.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.35~39( I-A-V-S-P) derived from Human Ring1A.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Ring1A protein.

Applications:

WB

Background:

Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

Cao R., Tsukada Y., Zhang Y.Mol. Cell 20:845-854(2005)
Satijn D.P.E., Gunster M.J., van der Vlag J., Hamer K.M. Mol. Cell. Biol. 17:4105-4113(1997)

References:

appl_detail:

Predicted MW: 58kd
Western blotting: 1:500~1:1000

Datasheet PDF