FoxA2(HNF3b) Antibody

Catalog Number:

21607

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9Y261
NCBI Protein: NP_068556.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.435~439( A-S-P-L-A) derived from Human FoxA2(HNF3b).

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total FoxA2(HNF3b) protein.

Applications:

WB

Background:

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' . In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.

Wolfrum C., Besser D., Luca E., Stoffel M. Proc. Natl. Acad. Sci. U.S.A. 100:11624-11629(2003)
Motallebipour M., Ameur A., Reddy Bysani M.S. Genome Biol. 10:R129.0-R129.0(2009)
Nock A., Ascano J.M., Jones T., Barrero M.J. J. Biol. Chem. 284:19915-19926(2009)

References:

appl_detail:

Predicted MW: 50kd
Western blotting: 1:500~1:1000

Datasheet PDF