PKM1 Antibody

Catalog Number:

21577

Amount:

100μg/100μl

Price:

$319

Swiss-Prot No:

P14618

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

The antiserum was produced against synthesized non-phosphopeptide derived from Human PKM1.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

PKM1 antibody detects endogenous levels of total PKM1 protein

Applications:

Background:

Pyruvate PKM1 encodes a protein involved in glycolysis, the protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. Pyruvate kinase isoform expressed in adult tissues, which replaces isoform M2 after birth [1] . The transition between the highly active tetrameric form and nearly inactive dimeric form contributes to the control of glycolysis and is important for tumor cell proliferation and survival [2,3,4] . In human cancer cells, epidermal growth factor receptor (EGFR) activation induces translocation of PKM2 into the nucleus, but PKM1 failed to translocate into the nucleus [5] .

References:

[1] Christofk HR, Vander Heiden MG, Harris MH, Ramanathan A, Gerszten RE, Wei R, Fleming MD, Schreiber SL, Cantley LC. The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Nature. 2008 Mar 13;452(7184):230-3. doi: 10.1038/nature06734. [2] Vander Heiden MG, Locasale JW, Swanson KD, Sharfi H, Heffron GJ, Amador-Noguez D, Christofk HR, Wagner G, Rabinowitz JD, Asara JM, Cantley LC. Evidence for an alternative glycolytic pathway in rapidly proliferating cells. Science. 2010 Sep 17;329(5998):1492-9. doi: 10.1126/science.1188015. [3] Dombrauckas JD, Santarsiero BD, Mesecar AD. Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis. Biochemistry. 2005 Jul 12;44(27):9417-29. doi: 10.1021/bi0474923. [4] Ashizawa K, McPhie P, Lin KH, Cheng SY. An in vitro novel mechanism of regulating the activity of pyruvate kinase M2 by thyroid hormone and fructose 1, 6-bisphosphate. Biochemistry. 1991 Jul 23;30(29):7105-11. doi: 10.1021/bi00243a010. [5] Yang W, Xia Y, Ji H, Zheng Y, Liang J, Huang W, Gao X, Aldape K, Lu Z. Nuclear PKM2 regulates β-catenin transactivation upon EGFR activation. Nature. 2011 Dec 1;480(7375):118-22. doi: 10.1038/nature10598.

appl_detail:

Predicted MW: 60kd
Western blotting: 1:1000

Datasheet PDF