ATR(Ab-428) Antibody

Catalog Number:

21505

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q13535
NCBI Protein: NP_001175.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.426~430 (G-I-S-P-K) derived from Human ATR.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total ATR protein.

Applications:

IHC

Background:

ATR encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.

Zhou, X.Z. et al. (1999) Cell Mol. Life Sci. 56, 788-806.
Pinna, L.A. and Ruzzene, M. (1996) Biochim. Biophys. Acta 1314, 191-225.
Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.
Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-908.

References:

appl_detail:

Predicted MW: 250kd
Immunohistochemistry: 1:50~1:100

Datasheet PDF