CD22 Antibody

Catalog Number:

21492

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P20273
NCBI Protein: NP_001762.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.839~843 (N-V-D-Y-V) derived from Human CD22.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total CD22 protein.

Applications:

WB

Background:

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to a-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Doody G.M., Justement L.B., Delibrias C.C., Matthews R.J., Lin J., Thomas M.L., Fearon D.T. Science 269:242-244(1995)
Tuscano J.M., Engel P., Tedder T.F., Agarwal A., Kehrl J.H.Eur. J. Immunol. 26:1246-1252(1996)
Law C.L., Sidorenko S.P., Chandran K.A., Zhao Z., Shen S.H., Fischer E.H., Clark E.A.J. Exp. Med. 183:547-560(1996)

References:

appl_detail:

Predicted MW: 140kd
Western blotting: 1:500~1:1000

Datasheet PDF