Vimentin Antibody

Catalog Number:

21488

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P08670
NCBI Protein: NP_003371.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.452~456(G-Q-V-I-N) derived from Human Vimentin.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Vimentin protein.

Applications:

WB IF

Background:

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

Sommers C.L., Walker-Jones D., Heckford S.E., Worland P., Valverius E., Clark R., McCormick F.,
Cancer Res. 49:4258-4263(1989)
Kang S.-M., Shin M.-J., Kim J.-H., Oh J.-W. Proteomics 5:2227-2237(2005)
Ahmed B.A., Bukhari I.A., Jeffus B.C., Harney J.T., Thyparambil S., Ziu E., Fraer M., Rusch N.J. PLoS ONE 4:E4730-E4730(2009)

References:

appl_detail:

Predicted MW: 57kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Datasheet PDF