EWS Antibody

Catalog Number:

21432

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9BZD1
NCBI Protein: NP_053733.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.213~217(T-Y-G-Q-P)derived from Human EWS.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of total EWS protein.

Applications:

WB

Background:

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.

Ubhi T.B.S., Rainey D.R., Craig A.R., Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases

References:

appl_detail:

Predicted MW: 85kd
Western blotting: 1:500~1:1000

Datasheet PDF