Lin28 Antibody

Catalog Number:

21426

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9H9Z2
NCBI Protein: NP_078950.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa. 1~5 (M-G-S-V-S) derived from Human Lin28

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Lin28 protein.

Applications:

WB

Background:

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells.

Heo I., Joo C., Mol. et al., Cell 32:276-284(2008)
Heo I., Joo C., Kim Y.-K., et al., Cell 138:696-708(2009)
Viswanathan S.R., Powers J.T., et al., Nat. Genet. 41:843-848(2009)

References:

appl_detail:

Predicted MW: 26kd
Western blotting: 1:1000

Datasheet PDF