Fas Antibody

Catalog Number:

21419

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P25445
NCBI Protein: NP_000034.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.323~327 (E-N-S-N-F) derived from human Fas .

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Fas protein.

Applications:

WB

Background:

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Hahne, M. et al. (1995) Int Immunol 7, 1381-6.
Nagata, S. (1997) Cell 88, 355-65.
Green, D.R. and Ferguson, T.A. (2001) Nat Rev Mol Cell Biol 2, 917-24.

References:

appl_detail:

Predicted MW: 40-50kd
Western blotting: 1:1000

Datasheet PDF