Smad3(Ab-425) Antibody

Catalog Number:

21325

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P84022
NCBI Protein: NP_001138574.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.423~427 (C-S-S-V-S) derived from Human Smad3.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Smad3 protein.

Applications:

WB IHC IF

Background:

Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Shi W, et al. J Cell Sci. 2007 Apr 1;120(Pt 7):1216-24
Seong HA, et al. J Biol Chem. 2007 Apr 20;282(16):12272-89
Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1191-200
LeClair RJ, et al. Circ Res. 2007 Mar 30;100(6):826-33

References:

appl_detail:

Predicted MW: 52kd

Western blotting1:500~1:1000

Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF