Smad2(Ab-220) Antibody

Catalog Number:

21323

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q15796
NCBI Protein: NP _001003652.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.218~222 (P-E-T-P-P) derived from Human Smad2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Smad2 protein.

Applications:

WB IHC

Background:

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420

References:

appl_detail:

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF