p56Dok-2(Ab-299) Antibody

Catalog Number:

21270

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O60496
NCBI Protein: NP_003965.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.297~301 (G-E-Y-A-V) derived from Human p56Dok-2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total p56Dok-2 protein.

Applications:

WB IHC IF

Background:

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation

Feng Cong, et,al. (1999) Mol. Cell. Biol; 19: 8314 - 8325.
Serge Lemay, et,al. (2000) Mol. Cell. Biol; 20: 2743 - 2754.
Ute Schaeper, et,al.(2000) J. Cell Biol; 149: 1419.
Miyuki Honma, et,al. (2006) Genes Cells; 11: 143 - 151.

References:

appl_detail:

Predicted MW: 56kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF