Cortactin(Ab-421) Antibody

Catalog Number:

21263

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q14247
NCBI Protein: NP_005222.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.419~423 (P-I-Y-E-D) derived from Human CORTACTIN.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Cortactin protein.

Applications:

IHC

Background:

Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Luo C, et al. (2006) J Biol Chem ; 281(40):30081-30093
Head JA, et al. (2003) Mol Biol Cell ;14(8):3216-3229
Salomon AR, et al. (2003) PNAS ; 100(2): 443-448
Huang C, et al. (1998) J Biol Chem ; 273(40): 25770-25776.

References:

appl_detail:

Predicted MW: 85kd
Immunohistochemistry: 1:50~1:100

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