Ephrin-B2(Ab-330) Antibody

Catalog Number:

21196

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P52799
NCBI Protein: NP_004084.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.328~332 (N-I-Y-Y-K) derived from Human Ephrin B (ephrin-B2).

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Ephrin-B2 protein.

Applications:

WB IF

Background:

Ephrin-B2 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a lycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.

Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al. (2006) Blood; 107(6):2330-2338.
Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101(15):5583-5588.

References:

appl_detail:

Predicted MW: 37kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Datasheet PDF