SMC1(Ab-957) Antibody

Catalog Number:

21190

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q14683
NCBI Protein: NP_006297.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa. 955~959 (G-S-S-Q-G) derived from Human SMC1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total SMC1 protein.

Applications:

WB IHC

Background:

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Yang XM. et al. (2004) Mol Cell Biol. 24(12): 5290-5303.
Kim ST. et al. (2002) Genes Dev. 16(5): 560-570.
Revenkova E.et al. (2001) Mol Cell Biol. 21(20): 6984-6998

References:

appl_detail:

Predicted MW: 145kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF