Zap-70(Ab-493) Antibody

Catalog Number:

21174

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P43403
NCBI Protein: NP _001070.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.491~495 (S-Y-Y-T-A) derived from Human ZAP70.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total Zap-70 protein.

Applications:

WB IHC

Background:

ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions

Chan AC, et al. (1995) EMBO J; 14(11): 2499-2508
Kong G, et al. (1996) Mol Cell Biol; 16(9): 5026-5035
Salomon AR, et al. (2003)Proc Natl Acad Sci U S A; 100(2): 443-448

References:

appl_detail:

Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF