cdc25A(Ab-76) Antibody

Catalog Number:

21163

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P30304
NCBI Protein: NP_001780.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa. 73~77 (M-G-S-S-E) derived from Human cdc25A.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total cdc25A protein.

Applications:

WB

Background:

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Jin J, et al. (2003) Genes 17(24): 3062-74.
Shimuta K, et al. (2002) EMBO J 21(14): 3694-703.

References:

appl_detail:

Predicted MW: 70kd
Western blotting: 1:500~1:1000

Datasheet PDF