p27Kip1(Ab-10) Antibody

Catalog Number:

21150

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P46527
NCBI Protein: NP_004055.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.8~12 (N-G-S-P-S) derived from Human p27Kip1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total p27Kip1 protein.

Applications:

WB IHC

Background:

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

Le XF, et al. (2003)J Biol Chem.
Connor MK, et al. (2003)Mol Biol Cell; 14(1): 201-13.
McAllister SS, et al. (2003) Mol Cell Biol; 23(1): 216-28.
Chopra S, et al. (2002)J Biol Chem; 277(36): 32413-6.
Boehm M, et al. (2002)EMBO J; 21(13): 3390-401.

References:

appl_detail:

Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF