eIF2a(Ab-51) Antibody

Catalog Number:

21271

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P05198
NCBI Protein: NP_004085.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.49~53 (E-L-S-R-R) derived from Human eIF2a.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total eIF2a protein.

Applications:

WB IHC

Background:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Xavier Saelens, et,al. (2001) J. Biol. Chem; 276: 41620 - 41628.
Hong-Li Wu, Yu-Hua Li, Yan-Hua Lin, et al. (2008) Salvianolic acid B protects human endothelial cells from oxidative stress damage: a possible protective role of glucose-regulated protein 78 induction Cardiovasc Res doi:10.1093/cvr/cvn262
This article references the use of the #21271.

References:

appl_detail:

Predicted MW: 38kd
Western blotting: 1:200~1:1000
Immunohistochemistry: 1:30~1:150
 

Datasheet PDF