G3BP-1(Ab-232) Antibody

Catalog Number:

21102

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q13283
NCBI Protein: NP_005745.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.230~234 (S-S-S-P-A) derived from Human G3BP-1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total G3BP-1 protein.

Applications:

WB IHC

Background:

May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

Tao WA, et al. (2005) Nat Methods. 2 (8): 591-598.
Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Beausoleil SA, et al. (2004) Proc Natl Acad Sci USA.101 (33): 12130-12135.
Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.

References:

appl_detail:

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF