MDM2(Phospho-Ser260) antibody

Catalog Number:

12171

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q00987
NCBI Gene ID: 4193

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

A phospho specific peptide corresponding to residues surrounding S450 of human MDM2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of MDM2 only when phosphorylated at serine 260.

Applications:

WB

Background:

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).

References:

appl_detail:

WB 1:500 - 1:2000

Datasheet PDF