Vitamin D3 Receptor (Phospho-Ser51) Antibody

Catalog Number:

12155

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P11473;
NCBI Gene#:7421

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 51 (R-R-S(p)-M-K) derived from Human Vitamin D3 Receptor.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Vitamin D3 Receptor only when phosphorylated at serine 51.

Applications:

WB

Background:

Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF