53BP1 (Phospho-Ser25) Antibody

Catalog Number:

12125

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:Q12888;
NCBI Gene#:7158

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 25 (E-D-S(p)-Q-P) derived from Human 53BP1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of 53BP1 only when phosphorylated at serine 25.

Applications:

IHC IF

Background:

Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation.

References:

appl_detail:

Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Datasheet PDF