Proteasome α3 (Phospho-Ser250) Antibody

Catalog Number:

12113

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P25788;
NCBI Gene#:5684

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 250 (D-E-S(p)-D-D) derived from Human Proteasome α3.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Proteasome α3 only when phosphorylated at serine 250.

Applications:

WB

Background:

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF