ERK8 (Phospho-Thr175/Tyr177) Antibody

Catalog Number:

12105

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:Q8TD08;
NCBI Gene#:225689

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 175 and tyrosine 177 (A-V-T(p)-E-Y(p)-V-A) derived from Human ERK8.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of ERK8 only when phosphorylated at threonine 175 and tyrosine 177.

Applications:

WB

Background:

In vitro, phosphorylates MBP.

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF