CDC25A (Phospho-Ser124) Antibody

Catalog Number:

12073

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P30304;
NCBI Gene#: 993;
NCBI Protein#: NP_001780.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 124 (S-H-S(p)-D-S) derived from Human CDC25A.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of CDC25A only when phosphorylated at Serine 124.

Applications:

WB

Background:

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

References:

appl_detail:

Predicted MW: 70kd
Western blotting: 1:500~1:1000

Datasheet PDF