RFA2 (Phospho-Thr21) Antibody

Catalog Number:

12060

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P15927;
NCBI Gene#: 6118;
NCBI Protein#: NP_002937.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Threonine 21 (G-Y-T(p)-Q-S) derived from Human RFA2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of RFA2 only when phosphorylated at Threonine 21.

Applications:

WB

Background:

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Ref.9 Ref.14 Ref.15 Ref.16 Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

References:

appl_detail:

Predicted MW: 35kd
Western blotting: 1:500~1:1000

Datasheet PDF