PI3-kinase p85- alpha (Phospho-Tyr607) Antibody

Catalog Number:

12057

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P27986;
NCBI Gene#: 5295;
NCBI Protein#: NP_001229395.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Tyrosine 607 (D-Q-Y(p)-S-L) derived from Human PI3-kinase p85-alpha.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of PI3-kinase p85- alpha only when phosphorylated at Tyrosine 607.

Applications:

WB IHC

Background:

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

References:

appl_detail:

Predicted MW: 80kd
Western blotting: 1:500~1:1000

Datasheet PDF