FOXO4 (Phospho-Thr451) Antibody

Catalog Number:

12053

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P98177;
NCBI Gene#: 4303;
NCBI Protein#: NP_001164402.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Threonine 451 (L-G-T(p)-P-V) derived from Human FOXO4.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of FOXO4 only when phosphorylated at Threonine 451.

Applications:

WB

Background:

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

References:

appl_detail:

Predicted MW: 65kd
Western blotting: 1:500~1:1000

Datasheet PDF