I kappaB- alpha (Phospho-Tyr305) Antibody

Catalog Number:

12050

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P25963;
NCBI Gene#: 4792;
NCBI Protein#: NP_065390.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Tyrosine 305(L-P-Y(p)-D-D) derived from Human I kappaB- alpha.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of I kappaB- alpha only when phosphorylated at Tyrosine 305.

Applications:

WB

Background:

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

References:

appl_detail:

Predicted MW: 39kd
Western blotting: 1:500~1:1000

Datasheet PDF