PTP1B (Phospho-Ser50) Antibody

Catalog Number:

12039

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P18031;
NCBI Gene#: 5770;
NCBI Protein#: NP_001265547.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 50(D-V-S(p)-P-F) derived from Human PTP1B.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of PTP1B only when phosphorylated at Serine 50.

Applications:

WB

Background:

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

References:

appl_detail:

Predicted MW: 49kd
Western blotting: 1:500~1:1000

Datasheet PDF