ADAM 17 (Phospho-Thr735) Antibody

Catalog Number:

12033

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P78536;
NCBI Gene#: 6868;
NCBI Protein#: NP_003174.3

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Threonine 735 (P-Q-T(p)-P-G) derived from Human ADAM 17.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of ADAM 17 only when phosphorylated at Threonine 735.

Applications:

WB

Background:

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.

References:

appl_detail:

Predicted MW: 93kd
Western blotting: 1:500~1:1000

Datasheet PDF